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Virtual Inseminator Pc [BEST]



Abstract: Simple SummarySmall-bodied live-bearing fishes attract broad attention because of their importance in biomedical research and critical conservation status in natural habitats. Artificial insemination is an essential approach used to establish hybrid lines and for the operation of sperm repositories. The existing mouth-pipetting technique for artificial insemination of live-bearing fishes has not been substantially upgraded since the first implementation in the 1950s. This work developed a low-cost standardized artificial inseminator device (SAID) as open hardware to address issues routinely encountered in insemination by mouth-pipetting, including lack of reproducibility among different users, difficulty in training, and large, unreportable variation in sample volume and pressure during insemination. Community-level enhancements of the SAID prototype could enable standardized insemination with minimal training and facilitate the participation of research communities in the use of cryopreserved genetic resources. AbstractSmall-bodied live-bearing fishes attract broad attention because of their importance in biomedical research and critical conservation status in natural habitats. Artificial insemination is an essential process to establish hybrid lines and for the operation of sperm repositories. The existing mouth-pipetting technique for artificial insemination of live-bearing fishes has not been substantially upgraded since the first implementation in the 1950s. The goal of this work was to develop a standardized artificial inseminator device (SAID) to address issues routinely encountered in insemination by mouth-pipetting, including lack of reproducibility among different users, difficulty in training, and large unreportable variation in sample volume and pressure during insemination. Prototypes of the SAID were designed as relatively inexpensive (




Virtual Inseminator Pc


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